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錘頭核酶的分子生物學機制

來源:解剖科學進展 作者:郭曉華 2004-9-29
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摘要: 摘要:近二十年來,錘頭核酶作為基因治療的一種重要RNA酶類越來越受到人們的廣泛關注,有關錘頭核酶抗病毒作用機理及應用等問題的研究正在逐步深入。本文僅就其分子結構、催化功能及影響核酶作用功效因素等分子生物學機制的研究進展作一綜述。 The structure, function and application of the hammerhead ribozyme。 Se......


  摘 要:近二十年來,錘頭核酶作為基因治療的一種重要RNA酶類越來越受到人們的廣泛關注,有關錘頭核酶抗病毒作用機理及應用等問題的研究正在逐步深入。本文僅就其分子結構、催化功能及影響核酶作用功效因素等分子生物學機制的研究進展作一綜述。參考文獻:

  [1]Birikh KR, Heaton PA, Eckstein F. The structure, function and application of the hammerhead ribozyme. Eur J Biochem, 1997, 245: 1

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  [3]Uhlenbeck OC. A small catalytic oligoribonucleotide. Nature, 1987, 328: 596

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  [10]Miller WA, Hercus T, Waterhouse PM. A satellite RNA of barley yellow dwarf virus contains a novel hammerhead structure in the selfcleaving domain. Virology, 1991, 183: 711

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  [13]Scott WG, Finch JT, Klug A. The crystal structure of an alRNA hammerhead ribozyme-a proposed mechanism for RNA catalytic cleavage. Cell, 1995, 81: 991

  [14]Tuschl T, Gohlke C, Jovin TM. A Three-dimensional model for the hammerhead ribozyme based on fluorescence measurements. Science, 1994, 266: 785

  [15]Burgin AB, Gonzalez Jr C, Karpeisky AM, et al. Chemically modified hammerhead rihozymes with improved catalytic rates. Biochemistry, 1996, 35: 14090

  [16]Kuimelis RG, McLaughlin LW. Probing the cleavage activity of the hammerhead ribozyme using analog complexes. Nuc Aci Mol biol, 1996a 10: 197

  [17]McKay DB. Structure and function of the hammerhead ribozyme an unfinished story. RNA, 1996, 2: 395

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  [19]Bassi GS, Murchie AIH, Lilley DMJ. The ion-induced folding of the hammerhead ribozyme: core sequence changes that preturb folding into the inactive conformation. RNA, 1996, 2: 756

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